Figure 5.

There are two main clusters of the BCL-2 enhancer marked by H3K27Ac and bound by P300 in MLL-AF4 and E2A-PBX1 cells. (A) ChIP-seq data as indicated in SEM cells. Red boxes highlight the two enhancer ATAC/H3K27Ac clusters. The yellow arrowhead indicates a PCR primer set used for ChIP Q-PCR BCL-2 enhancer experiments in Figure 6. (B) ChIP-seq in RCH-ACV (E2A-PBX1) cells reveals binding of E2A-PBX1 and P300 at the two enhancer clusters.