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. 2017 Feb 28;5:e2961. doi: 10.7717/peerj.2961

Figure 2. Size exclusion chromatography (SEC) and SDS-PAGE profile of PmSTS.

Figure 2

(A) SEC and SDS PAGE profile of PmSTS without presence of glycerol in the purification buffer. (B) SEC and SDS PAGE profile of PmSTS in the presence of 15% (v/v) glycerol in the purification buffer. (C) SEC and SDS PAGE profile of truncated PmSTS_Δ24 in the presence of 15% (v/v) glycerol in the purification buffer. M, protein molecular weight markers (kDa); 1, Soluble fraction of uninduced cell lysate; 2, Soluble fraction of induced cell lysate; 3, Protein purified by immobilized metal affinity chromatography (IMAC); 4, Protein fraction from peak 4 in SEC; 5, Protein fraction from peak 5 in SEC.