Adipose tissue protein activation related to lipid metabolism in obese non-GDM, GDM, and nonpregnant controls. Adipose tissue was obtained from subcutaneous abdominal areas and Western blots were performed for DGAT1 (A), phospho-Perilipin Ser522
(B), phospho-HSL Ser660 (C), ATGL (D), phospho-AMPKα Thr172 (E), and phospho-ACC Ser79
(F). Data reported as mean ± SEM in the bar graphs. Statistical analysis was performed using Kruskal–Wallis test with Dunn's multiple comparisons. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein kinase; ATGL, adipose triglyceride lipase; DGAT1, diacylglycerol acyltransferase 1; HSL, hormone-sensitive lipase.