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. 2017 Mar 2;7:43655. doi: 10.1038/srep43655

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Transmission electron micrographs of 3 hours 5 mM MTZ treated Cherry larvae showed appearance of pseudocysts (arrowheads) and numerous endosomes (arrows) close to the pseudocysts (lumen marked with asterisk). Additionally those larvae showed broad effacement of foot processes. In contrast, control treated larvae (0.1% DMSO) showed normal glomerular morphology with regular foot processes and slit diaphragms in between. Note the electron dense Bowman’s space (BS) in MTZ treated larvae (scale bars represent 1 μm, representative images from n = 3 experiments).