Skip to main content
. Author manuscript; available in PMC: 2017 Mar 2.
Published in final edited form as: Biochem J. 2015 Jun 29;470(2):181–193. doi: 10.1042/BJ20141494

Figure 2. ERRγ is involved in 2-AGE-mediated induction of CYP7A1 gene expression.

Figure 2

(A and B) AML12 cells were infected with adenovirus US (Ad-US) or adenovirus sh-ERRγ (Ad-shERRγ) for 48 h followed by treatment with 2-AGE (10 µM). Cell culture media were collected to determine bile acid levels (A, right panel). ***P < 0.001. (C and D) AML12 cells were infected with Ad-GFP (control), Ad-ERRα and Ad-ERRγ . ***P < 0.001. (E and F) HepG2 cells were infected with Ad-GFP (control), Ad-ERRα and Ad-ERRγ. Total RNA and protein in (A–F) were isolated and used for qPCR and Western blot analysis. ***P < 0.001. All data are representative of at least three independent experiments. Error bars show ± S.E.M.