Skip to main content
. Author manuscript; available in PMC: 2017 Mar 2.
Published in final edited form as: Biochem J. 2015 Jun 29;470(2):181–193. doi: 10.1042/BJ20141494

Table 1.

List of primers, forward primer (F) and reverse primer (R), used for amplification using qPCR and site-directed mutagenesis

Name Sequence(5′-3′)
ERRE1 MUT F TGCCTTCCAAGGCAATATTTCCAATCCTCTCTCCAC
ERRE1 MUT R GTGGAGAGAGGATTGGAAATATTGCCTTGGAAGGCA
ERRE2 MUT F CCTACTGCTTCTGCTATTTGACCTGAGAGGGTCG
ERRE2 MUT R CGACCCTCTCAGGTCAAATAGCAGAAGCAGTAGG
m-CYP7A1 F TCTCAAGCAAACACCATTCCT
m-CYP7A1 R GGCTGCTTTCATTGCTTCA
m-CYP27A1 F CCTCACCTATGGGATCTTCATC
m-CYP27A1 R TTTAGGCATCCGTGTAGAGC
m-CYP7B1 F AATTGGACAGCTTGGTCTGC
m-CYP7B1 R TTCTCGGTGATGCTGGAGT
m-CYP8B1 F GCAGCACTGAATACCCATCC
m-CYP8B1 R TCTGAGAGCTGGGGAGAGG
h-CYP7A1 F GCATCATAGCTCTTTACCCAC
h-CYP7A1 R GGTGTTCTGCAGTCCTGTAAT
m-ERRγ F GGATGGGCAAAACATATTCC
m-ERRγ R ACAACGCCGAGGACTCAGA
m-SHP F CACCTGCATCTCACAGCCACT
m-SHP R GCCAACCCAAGCAGGAAGA
r-CYP7A1 F TGCTCTGTGTTCACTTTCTG
r-CYP7A1 R ACTCGGTAACAGAAGGCATA
r-actin F GGCACCACACTTTCTACAAT
r-actin R AGGTCTCAAACATGATCTGG
m-cb1R F GGGCAAATTTCCTTGTAGCA
m-cb1R R GGCTCAACGTGACTGAGAAA
m-LRH-1-F TTG AGT GGG CCA GGA GTA GT
m-LRH-1-R TCG GTA AAT GTG ATC GAG AAT C
m-HNF4α-F GCC AAG ATT GAC AAC CTG CT
m-HNF4α-R CCC ATG TGT TCT TGC ATC AG
m-LXRα-F AGA GCC TCC AGG GTG AGG
m-LXRα-R AGC CCT GGA CAT TAC CAA GA