Figure 4. Effect of estrogen receptors (ERs) activator with 17β-estradiol (E2) incubation on semaphorin (sema) 4D expression and cell proliferation. A, sema 4D m-RNA expression by RT PCR. B, sema 4D protein expression by western blotting. β-actin was used as the internal standard. C, Cell proliferation assay. Data are reported as relative viability of cells represented by the ratio of OD₄₅₀ values. Experiments were carried out in triplicate. Data are reported as means±SD (*P<0.05, **P<0.01, ANOVA).