Figure 5. Effect of estrogen receptors (ERs) inhibitor ICI182-780 on semaphorin (sema) 4D expression and cell proliferation. A, sema 4D protein expression by western blotting; B, sema 4D m-RNA expression by RT PCR. β-actin was used as the internal standard; C, cell proliferation assay. Data are reported as means±SD of relative viability of cells represented by the ratio of OD₄₅₀ values.