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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Exp Cell Res. 2016 Aug 15;347(2):301–311. doi: 10.1016/j.yexcr.2016.08.009

Figure 3. Phagocytosis was enhanced in cells treated with PDGF or transfected with Rac1-61L.

Figure 3

(A) TM-1 cells treated with 60ng/ml PDGF during the duration of the phagocytic assay showed a statistically significant (* p<0.05) increase in the number of cells that took up the pHrodo bioparticles compared to untreated control cells. (B) Overexpression of a CA-Rac1-61L also caused a significant increase (**p<0.01) in the uptake of pHrodo bioparticles compared to mock transfected cells, cells transfected with DN-Rac1-N17, or cells transfected with an empty pcDNA3 vector. Phagocytic activity of TM-1 cells was measured after 4 hr challenge with the pHrodo bioparticles in the presence of 60ng/ml PDGF and assessed by counting the number of cells containing ≥3 pHrodo bioparticles. The phagocytic activity is expressed as the percentage of bioparticle positive cells (BPC) relative to mock transfected control. Histograms are representative of the results obtained in three independent experiments as described in materials and methods.