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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Exp Cell Res. 2016 Aug 15;347(2):301–311. doi: 10.1016/j.yexcr.2016.08.009

Figure 4. Expression of factors involved in Rac1 activation in TM-1 cells.

Figure 4

(A) RT-PCR analysis showed that TM-1 cells expressed mRNA for the GEFs Vav2, Tiam1 and Tiam2. RNA was normalized to SDHA and expressed relative to Tiam1 mRNA levels. (B) RT-PCR showed that RhoG, Dock 180 and ELMO2 mRNA could be detected in TM-1 cells. Neither ELMO1 nor ELMO3 mRNA could be detected (not shown). Level of Rac1 mRNA was used as a positive control. RNA was normalized to SDHA and expressed relative to RhoG mRNA levels. All experiments were done using triplicate determinations and the histograms shown are representative of three independent experiments.