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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Exp Cell Res. 2016 Aug 15;347(2):301–311. doi: 10.1016/j.yexcr.2016.08.009

Figure 8. Knock down of ILK impairs phagocytosis.

Figure 8

(A) Quantification of the number of cells transfected with Dock1 siRNA that took up pHrodo bioparticles. (B) The level of DOCK1 mRNA expression was significantly reduced in TM-1 cells transfected with Dock1 siRNA compared to cells transfected with a NT siRNA (*p<0.05). RNA was normalized to SDHA and expressed relative to Dock1 mRNA level in Mock transfected cells. The histogram is representative of the results obtained from three independent experiments. All experiments were done as described for Figure 6 using triplicate determinations. (C) The number of cells that took up pHrodo bioparticles was reduced in cells transfected with siRNA to ILK compared to cells transfected with non-targeting siRNA. The histograms shown (A & C) are representative of the results obtained from three independent experiments. Phagocytosis was quantified by counting the number of cells that had taken up ≥3 pHrodo bioparticles and expressed as %BPC ± SEM relative to NT control as described for Figure 6.