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. 2017 Mar 2;37(2):BSR20160519. doi: 10.1042/BSR20160519

Figure 1. STIM1 was expressed and knocked down in human tongue squamous carcinoma cells.

Figure 1

(A) Expression of STIM1 mRNA was measured by qRT-PCR in two tongue squamous carcinoma cell lines, Tca-8113 and CAL27. The relative expression of STIM1 is shown as ΔCT (CTSTIM1 CTGAPDH). (B) Representative image showing lentivirus infection in Tca-8113 cells. Tca-8113 cells were infected with shSTIM1 and shCtrl lentivirus for 48 h, and then the efficiency of infection was assessed by light microscopy and fluorescent microscopy at the third day infection. (C) Knockdown of STIM1 in Tca-8113 cells. Tca-8113 cells were infected with shSTIM1 and shCtrl lentivirus for 48 h, and then qRT-PCR analysis was performed to analyze the mRNA level of STIM1 in Tca-8113 cells; ***P<0.001.