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. 2017 Apr;443:18–25. doi: 10.1016/j.jim.2017.01.008

Table 1.

Covalent binding of αIL-10 neutralizing antibody to NHS coated magnetic beads leads to minimal residual αIL-10 antibody in depleted ascites samples.

Samples αIL-10 antibody IgG2b isotype
#1 1.3 ng/ml 2.0 ng/ml
#2 0.8 ng/ml 2.3 ng/ml
#3 0.5 ng/ml 2.5 ng/ml
#4 0.6 ng/ml 2.5 ng/ml

Levels of mouse IgG2b antibody were measured in four individual ascites samples after incubation with NHS magnetic beads bound to αIL-10 or isotype control antibody. The mouse IgG2b concentration was quantified by sandwich ELISA.