Table 1.
Samples | αIL-10 antibody | IgG2b isotype |
---|---|---|
#1 | 1.3 ng/ml | 2.0 ng/ml |
#2 | 0.8 ng/ml | 2.3 ng/ml |
#3 | 0.5 ng/ml | 2.5 ng/ml |
#4 | 0.6 ng/ml | 2.5 ng/ml |
Levels of mouse IgG2b antibody were measured in four individual ascites samples after incubation with NHS magnetic beads bound to αIL-10 or isotype control antibody. The mouse IgG2b concentration was quantified by sandwich ELISA.