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Covalent binding of αPGE2 neutralizing antibody to NHS magnetic beads leads to minimal residual αPGE2 antibody in depleted ascites samples.
| Samples | αPGE2 antibody | IgG1 isotype |
|---|---|---|
| #1 | 1.3 ng/ml | 0.4 ng/ml |
| #2 | 1.3 ng/ml | 0.2 ng/ml |
Levels of mouse IgG1 αPGE2 neutralizing antibody and respective isotype control antibody were measured in ascites samples after depletion of PGE2 using NHS magnetic beads. The mouse IgG1 concentration was quantified by sandwich ELISA.
