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. 2017 Apr;443:33–44. doi: 10.1016/j.jim.2017.01.010

Fig. 4.

Fig. 4

IgG glycosylation state differences captured by lectin detection reagents.

A) Signal to noise ratios of lectin detection reagents for antibody samples from HIV + subjects (N = 11) against a technical negative (blank) on anti-huIgG and gp120 YU2 conjugated beads. Shaded region denotes values with a signal:noise ratio of < 2. B) Signal to noise ratios of AAL lectin detection for HIV positive and negative subjects (N = 3) on gp120 CN54 beads with and without prior PNGase treatment. Shaded region denotes values with a signal:noise ratio of < 2. C) Overview of PNGase treatment of conjugated beads. Signal of HIVIG compared to a technical negative (PBS) sample against beads conjugated with the named antigens before and after PNGase treatment and detected with tetramerized lectin detection reagents. D–F) Observed signal intensities (MFI) for a set of twelve subject samples with similar responses against gp120 CN54 when detected with total anti-huIgG-PE (D) but differing response magnitude when assayed with the fucose-specific lectin detection reagent LCA (E), and the sialic acid-sensitive lectin SNA (F). G) Correlation between signals (MFI) from fucose-specific lectin detection reagents (AAL and LCA) on anti-huIgG beads and the prevalence of fucosylated glycoforms from total serum IgG for a set of 10 subject samples.