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. 2017 Mar 2;12(3):e0168638. doi: 10.1371/journal.pone.0168638

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© 2017 Watanabe et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — a. Paraffin-embedded sections of the kidneys infected with CAG-EGFP adenovirus. The arrow indicates glomerulus. Adenovirus-derived EGFP was stained in brown. b and c. Paraffin embedded sections of the kidney infected with NKCC2DI-EGFP adenovirus. Adenovirus-derived EGFP is shown in brown and endogenous NKCC2 is shown in pink. e and f. Immunostaining of frozen kidney sections infected with CAG-EGFP is shown in e and NKCC2DI-EGFP is shown in f. e.Green indicates CAG promoter derived EGRP and f. Green indicates NKCC2 promoter-derived EGFP. f. Red indicates endogenous NKCC2. e and f. Blue indicates nucleus.