Table 3. Benchmarking results.

Analyses were performed on a set of 7000 regions composed of different human target types (see Methods for details). % full depth refers to the percent of targets receiving n = 10 sgRNA pair designs. % partial depth refers to targets receiving 0<n<10 designs. Designed targets refers to the total number of target features receiving full or partial depth designs.

Name	Non-default parameter	Wallclock Time (s)	% full depth	% partial depth	Mean pairs per target	Mean protospacer score	Mean pair score	Mean pair distance	Total sgRNA pairs	# Input targets	# Designed targets
Default		2639	68	18	8	0.52	1.04	1375	53760	7000	6024
Diversity0.1	v = 0.1	4748	0	86	1	0.62	1.25	1374	6024	7000	6024
Diversity 1	v = 1	4764	70	16	9	0.52	1.04	1375	54868	7000	6024
off1	t = 1,0,1,x,x	4869	83	9	9	0.57	1.15	1452	60959	7000	6417
off2	t = 1,0,2,x,x	4783	87	7	9	0.6	1.19	1451	62722	7000	6519
off3	t = 1,0,3,x,x	4802	88	6	9	0.6	1.20	1451	63514	7000	6564
off4	t = 1,0,4,x,x	4827	89	5	9	0.61	1.21	1450	63959	7000	6592
off5_1	t = 1,1,5,x,x	4883	90	5	9	0.6	1.21	1446	65030	7000	6695
pScore0.6	sp = 0.6	4878	63	23	8	0.53	1.06	1366	51488	7000	5974
pScore1	sp = 1	4691	29	41	7	0.6	1.20	1331	32674	7000	4881
pScore1.2	sp = 1.2	5318	9	42	2	0.67	1.33	1252	17226	7000	3587
PScore1.6	sp = 1.6	4658	0	7	0	0.83	1.66	1130	849	7000	513
design_2000	du = 2000 dd = 2000	9266	95	2	9	0.70	1.40	2874	67265	7000	6781