Fig 3. Up-regulated IFN-γ inhibits HCV replication in mice treated with human PBMCs and IFN-α.

Mice were treated as described in the legend to Fig 2. (a) Time course of serum IFN-γ concentration in mice treated with or without IFN-α. Mice treated with IFN-α without PBMC injection were also analyzed. Data are presented as the mean ± SD. (b) Liver mononuclear cells were stained with antibodies against human CD3 and CD56, human TCR Vα24, and TCR Vβ11 and analyzed by flow cytometry (upper panel). The frequency of IFN-γ-positive cells in NKT cells, and type I NKT cells were analyzed (lower panel). (c) Twenty-four hrs after IFN-α injection, intrahepatic gene expression levels of HLA-DMB and GTP-binding protein 5 (GBP5) were measured. RNA levels are expressed relative to β-actin mRNA. Data are represented as the mean ± SD of 3 mice.