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. 2017 Mar 2;12(3):e0172947. doi: 10.1371/journal.pone.0172947

Fig 3. Medial pathway neural precursors undergo precocious and supernumerary differentiation into neurons in baz1 mutants.

Fig 3

Confocal images of developing trunk DRGs of WT (A, D, G, J), baz1 (B, E, H, K) and m618 mutants (C, F, I, L) showing Elav1/Hu (red) and sox10:GFP (green) at each of 36 (A-C), 42 (D-F), 48 hpf (G-I) and 5 dpf (J-L). Arrowheads indicate subset of Elav1/Hu+ DRG sensory neurons. M-P) Counts (mean±s.d.) of trunk (Tr) and tail (Ta) and total (TOT) Elav1+ cells in DRGs of baz1 (yellow) and m618 (blue) mutants and their respective WT siblings. Significantly elevated numbers of neurons are indicated (two-tailed Student’s t test; **, p<0.01; ***, p<0.001). Note in panels J-L) that variable prominence of Elav1/Hu detection in spinal cord is an artefact of antibody penetration into CNS at this late stage. Scale bar, 50 μm.