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. 2017 Jan 23;8:6. doi: 10.3389/fimmu.2017.00006

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Copyright © 2017 Carlini, Picard, Garulli, Piquemal, Roubertoux, Chiaroni, Chanez, Gras and Di Cristofaro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HLA-G protein expression in human bronchial epithelium cell lysates from healthy controls (HC), patients with mild asthma (MA), and patients with severe asthma (SA). Semi-quantitative analysis (A.U., arbitrary units, mean and range) of Western Blot 4H84 staining normalized with GAPDH (mAb clone 9545) staining in HC [N = 10; 0.231 (0.137–0.552)], MA [N = 9; 0.186 (0.06–0.291)] and SA [N = 9; 0165 (0.004–0.492)]. The bands observed at 39 and 37 kDa may correspond to the biggest open reading frame from nucleotide 1 of HLA-G1 and HLA-G5 mRNA, respectively. Kruskal–Wallis ANOVA (HC, MA, SA) p = 0.214, non-significant; Mann–Whitney (HC vs. MA and SA) p = 0.088, non-significant.