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A-B: Cell viability of BPH-1 (A) and P69 (B) 24 hours after treatment with various doses of metformin. MTS test was performed as described in Materials and Methods. The results are expressed as percent of viable cells compared with control group. C-D: Cell viability of BPH-1 (C) and P69 (D) after the treatment of 5 mM metformin in different times. *, P<0.05; **, P<0.001 compared with control group.
