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. 2017 Mar 2;12(3):e0173335. doi: 10.1371/journal.pone.0173335

Fig 5. Metformin regulates cell cycle and the expression of proteins regulating cell cycle.

Fig 5

A: Representative immunoblot analysis of cyclin D1, D2 and D3 in BPH-1 cells 48 hours after treatment of indicated dose of metformin. GAPDH was used as a loading control. B: The effect of 48-hour treatment of 100 ng/mL IGF-1 and 5 mM metformin on the expression of cyclin D1, D2 and D3 in BPH-1 cells. C-D: Flow cytometry analysis of proliferating BPH-1 (C) and P69 (D) cells 48 hours after the addition of 100 ng/mL IGF-1, 5 mM metformin or both. E-F: The population of G0/G1 cells in BPH-1 (E) and P69 (F) cells (n = 3). *, P<0.05 compared with the control group; #, P<0.05 compared with the IGF-1 treated group. CTR: non-treated control, Met: metformin.