Fig 2. Effect of β-alanine and L-aspartate on the P3HP production.

The strain Q2153 was grown in minimal medium with supplement of 5 g/L β-alanine (β-Ala) or L-aspartate (L-Asp), cultivation without amino acid was used as the control (CK), and the P3HP production (white), CDW (light grey), and P3HP content (heavy grey) were presented. The experiment was carried out in shaking flask in triplicate. All shake-flask experiments were incubated for 48 h after induction.