Table 2. Effect of IPTG on the biomass and P3HP production.
Experiments | IPTG (mM) | Methods | CDW (g/L) | P3HP (g/L) | Content (%) |
---|---|---|---|---|---|
1 | 0.025 | Once | 6.67 ± 0.13 | 0.17 ± 0.01 | 2.55 |
2 | 0.025 | Every 24h* | 6.71 ± 0.15 | 0.39 ± 0.06 | 5.81 |
3 | 0.025 | Every 12h** | 5.68 ± 0.20 | 0.34 ± 0.01 | 5.99 |
4 | 0.05 | Once | 7.51 ± 0.37 | 0.73 ± 0.05 | 9.72 |
5 | 0.05 | Every 24h* | 7.01 ± 0.12 | 0.98 ± 0.02 | 13.98 |
6 | 0.05 | Every 12h** | 5.63 ± 0.14 | 0.97 ± 0.07 | 17.23 |
7 | 0.10 | Once | 4.46 ± 0.28 | 0.66 ± 0.03 | 14.80 |
8 | 0.10 | Every 24h* | 4.05 ± 0.09 | 0.71 ± 0.10 | 17.53 |
9 | 0.10 | Every 12h** | 2.95 ± 0.08 | 0.52 ± 0.04 | 17.63 |
*supplemented at 24h after the first time,
** supplemented at 12h, 24h and 36h after the first time