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. 2017 Mar 2;13(3):e1006185. doi: 10.1371/journal.ppat.1006185

Fig 3. qRT-PCR expression analysis of Sc-flp-21 and off-target control gene.

Fig 3

(A) Sc-flp-21 transcript ratio relative to Sc-act following 24 h incubation in 5 mg/ml dsRNA, 50 mM serotonin. (B) Sc-flp-21 transcript ratio relative to Sc-act following 24 h incubation in 5 mg/ml dsRNA, 50 mM serotonin, and 24 h in PBS; (C) Sc-flp-21 transcript ratio relative to Sc-act following 48 h incubation in 5 mg/ml dsRNA, 50 mM serotonin; (D) Sc-flp-21 transcript ratio relative to Sc-act following 48 h incubation in a reduced 1 mg/ml dsRNA, 50 mM serotonin; (E) L596_g5821.t1 transcript ratio relative to Sc-act following 48 h incubation in 5 mg/ml dsRNA, 50 mM serotonin. *P<0.05; **P<0.01; ****P<0.0001.