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. 2016 Sep 14;95(5):95. doi: 10.1095/biolreprod.116.142372

FIG. 1.

FIG. 1

Expression of SIRT3 in myometrium. A and B) Human myometrium was obtained from nonlaboring (term no labor, n = 8 patients) and laboring women at term who underwent cesarean section (term in labor, n = 8 patients). A) SIRT3 mRNA expression was analyzed using qRT-PCR and fold change was calculated relative to term no labor group. Data are mean ± SEM. *P < 0.05 versus term no labor (Student t-test). B) SIRT3 protein expression was analyzed by using Western blotting and fold change was calculated relative to term no labor group. Data are mean ± SEM. *P < 0.05 versus term no labor (Student t-test). Representative Western blots from 4 patients per group are also shown. C and D) Human primary myometrial cells were treated with or without 1 ng/ml IL1B or 10 ng/ml TNF for 20 h (n = 5 patients per treatment). C) SIRT3 mRNA expression was analyzed by using qRT-PCR, and fold change was calculated relative to basal level. Data are mean ± SEM. *P < 0.05 versus basal (paired sample t-test). D) SIRT3 protein expression was analyzed by using Western blotting and the fold change was calculated relative to basal. Data are mean ± SEM. *P < 0.05 versus basal (paired sample t-test). Representative Western blot from 1 patient is also shown.