FIG. 4.
Effect of siSIRT3 on the PTGS2-prostaglandin pathway. Human primary myometrial cells were transfected with 50 nM siCONT or 50 nM siSIRT3 for 48 h and then treated with 1 ng/ml IL1B (A–C) or 10 ng/ml TNF (D–F) for an additional 24 h (n = 5 patients). A, B, D, and E) PTGS2 and PTGFR mRNA expression levels were analyzed by using qRT-PCR. C, F) PGF2α concentration in the incubation medium was assayed using ELISA. For all data, the fold change was calculated relative to IL1B-stimulated siCONT-transfected cells and displayed as mean ± SEM. *P < 0.05 versus IL1B-stimulated siCONT-transfected cells (one-way ANOVA); **P < 0.05 versus TNF-stimulated siCONT-transfected cells (one-way ANOVA).