FIG. 7.

Effect of siSIRT3 on NFκB1 activation. Human myometrial cells were transfected with 300 ng/ml NFκB1 reporter construct. After 6 h, cells were transfected with 50 nM siCONT or 50 nM siSIRT3 for 48 h and then treated with 1 ng/ml IL1B (A) or 10 ng/ml TNF (B) for an additional 20 h (n = 5 patients). The fold change was calculated relative to that of IL1B- or TNF-stimulated siCONT-transfected cells. All data are mean ± SEM. *P < 0.05 versus IL1B-stimulated siCONT-transfected cells (one-way ANOVA); **P < 0.05 versus TNF-stimulated siCONT-transfected cells (one-way ANOVA).