Figure 5. The percentage methylation of the Treg-specific demethylated region (TSDR) between ALS patients’ and controls’ Tregs.

(A) The percentage TSDR methylation combining all 15 CpG islands among the 3 groups. ALS patients exhibited more methylation in the TSDR of Tregs compared with the TSDR of slowly progressing ALS patients and the control group. Significance determined by Student’s t test. (B) The percentage TSDR methylation of each individual CpG island among the 3 groups. The TSDR methylation was especially enhanced in islands 12 through 15 in the rapidly progressing ALS patients compared with slowly progressing ALS patient and the control group. There were no differences across all 15 CpG island between slowly progressing ALS patients and the control group.