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. 2017 Mar 9;2(5):e90780. doi: 10.1172/jci.insight.90780

Figure 3. Inflammatory cells play an important role in cholestatic liver injury in both mice and humans.

Figure 3

(A) Representative H&E–stained liver sections from Ccl2+/+ and Ccl2–/– mice after bile duct ligation (BDL) for 7 days (left). Hepatocyte necrosis is significantly reduced in Ccl2–/– mice after 7 days of BDL (right), as assessed by quantitative analysis of liver sections (mean ± SD, *P < 0.05, n = 6–7). Original magnification, ×40. (B) A pair of representative flow cytometry plots of neutrophils (CD11b+ and Gr1+ cells) in Ccl2+/+ and Ccl2–/– livers after BDL is on the left. Neutrophils were significantly reduced (right) in livers of Ccl2–/– mice after 7-day BDL. **P < 0.01, n = 3. (C) Immunoperoxidase stain (left) obtained from a representative cholestatic patient liver biopsy showing many portal and lobular myeloperoxidase-positive cells (original magnification, ×200). Hepatic injury (serum alanine aminotransferase [ALT]) positively correlated with periportal (middle) but not lobular (right) neutrophil infiltration in the liver of cholestatic patients. Statistical significance in A and B determined by 2-tailed Student’s t test.