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. 2017 Feb 26;23:39–51.

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Copyright © 2017 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Verification with LCM-RT–PCR of the lack of epithelial contamination in −4.5D PRK, −9.0D PRK, or unwounded control corneas. cDNA of laser capture microdissection (LCM) fragments cut from the anterior stroma of each cornea (as shown in Figure 2) were analyzed using quantitative real-time PCR for keratocan (a keratocyte marker) and keratin 3 (K3) and keratin 12 (K12; epithelial cell markers). Keratocan (keratocytes), but not K3 or K12, mRNA was detected in all three types of anterior stromal samples. Any cDNA sample that showed K3 or K12 contamination was discarded, and cDNA was prepared from newly cut LCM fragments.