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. 2017 Feb 26;23:39–51.

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Copyright © 2017 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Analysis of EBM component mRNAs in the anterior stroma at 2 days after PRK with quantitative real-time PCR. Nidogen-1 (Nid-1) mRNA was not statistically significantly increased in the anterior stroma of −4.5D photorefractive keratectomy (PRK) or −9.0D PRK corneas compared with the unwounded control corneas at this early time point. The nidogen-2 (Nid-2), perlecan, and laminin α-3 (LAMA3) mRNAs were statistically significantly increased in the anterior stroma of the −4.5D PRK and −9.0D PRK corneas compared to the unwounded control corneas. The Nid-2 and LAMA3 mRNAs were statistically significantly increased in the anterior stroma at 2 days after surgery in the −9.0D PRK corneas compared with the −4.5D PRK corneas. (n = 4 for each group; ***, p<0.01; **`).