Table 1. PCR primers designed for LCM-RT–PCR.
| Gene | Primer sequences (5’-3’) | Size (bp) | Annealing (°C) | GenBank accession |
|---|---|---|---|---|
|
NID1 |
F: ATGGGTTCAGCATCACTGGG
R: TAATGACCAGCTTGCCTGGG |
81 |
58 |
XM_017347600.1 |
|
NID2 |
F: CGTGCAAGACACGGAAGTCA
R: GAGTTGGCTGGGACGTAAGG |
141 |
58 |
XM_008269653.2 |
|
HSPG2 |
F: CGTGGCAGTCAACACCAAAG
R: TTCTTGATGCAGCCCGTGAT |
105 |
58 |
XM_017346486.1 |
|
LAMA3 |
F: CTCCCAAATGCCTCGTTCCT
R: TTTGTACCGCACCCTGAGAC |
138 |
58 |
XM_002713604.1 |
|
KERA |
F: AGTGCGGATGACTTTGATTG
R: TGGCTTCTCTGGAATGGTTT |
165 |
58 |
XM_002711384.3 |
|
KRT12 |
F: GGCTCTCTGTATCTCCCCTCT
R: CCATTTCCACGCTGACCT |
541 |
59 |
XM_002719366.3 |
|
KRT3 |
F: ATGTCCGTGGTGCTGTCC
R: GCTGGCGTTCTGCTTCTT |
267 |
60 |
XM_002711005.2 |
| ACTB | F: CACCCTCTCTCGACGAAACC R: CTGTCACCTTCACCGTTCCA | 152 | 60 | NM_001101683.1 |