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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Nature. 2017 Feb 8;543(7643):122–125. doi: 10.1038/nature21356

Fig. 3. The most common focal amplifications in cancer are contained on ECDNA.

Fig. 3

a, Comparison of the frequency of focal amplifications detected by next generation sequencing of 117 cancer samples studied here (blue), with those of matched tumor types in the TCGA (red), demonstrates significant overlap and representative sampling (p-value 10−6 based upon random permutations of TCGA amplicons; Methods). b, Localization of oncogenes by FISH. c, Representative FISH images of focal amplifications on ECDNA (arrows). d, EGFRvIII and c-Myc mRNA level, measured by qPCR (p < 0.001, Mann-Whitney test), mean ± SEM. n=17; each data point represents qPCR values from three technical replicates.