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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Cancer Res. 2016 Dec 28;15(3):340–347. doi: 10.1158/1541-7786.MCR-16-0287-T

Figure 1. Altering HER2 expression changes PARP1 but not PARP2 protein levels.

Figure 1

(A, B) Cells were transfected with HER2 or control (CON) siRNA for 72 hours and then analyzed by Western blot analysis for HER2, PARP1, and PARP2 protein levels. β-actin was used as a loading control. MDA-MB-231 cells that (C) transiently or (D) stably express the HER2 vector (231 HER2) as compared to cells expressing the control plasmid (231 NEO) were subjected to Western blot analysis for HER2, PARP1, PARP2, and β-actin. Data shown are representative immmunoblots from one of two independent experiments.