Fig. 5.
A2A increases mRNA levels of VEGF. HUVECs were isolated from normal and pre-eclamptic pregnancies. a Representative image of semiquantitative PCR for A2A, VEGF, and their respective loading control 18S. Cells from normal pregnancies (1 to 4) and cells pre-eclamptic pregnancies (5 to 8). Lines 1 and 5, in the absence of CGS-21680 or SNAP; 2 and 6, in the presence of CGS-21680; 3 and 7, in the presence of CGS-21680 and siRNA-A2A; 4 and 8, in the presence of CGS-21860, SNAP and siRNA-A2A. b Densitometry of A2A/18S ratio in normal cells (white bars) and pre-eclamptic (hatched bars) that were co-incubated with CGS and/or SNAP and/or siRNA-A2A, whose presence (+) and/or absence (−) is marked with respective symbols. c Densitometry of VEGF/18S ratio in normal and pre-eclampsia-derived cells as described in b. The results are expressed as medians and interquartile ranges. In b, *p < 0.05 vs cells from normal pregnancy at baseline. †p < 0.05 vs cells from pre-eclampsia at baseline. In c, *p < 0.05 vs cells from normal pregnancy at baseline. ‡p < 0.05 vs CGS-21680 in normal pregnancy. n = 4 for each group in duplicate