Fig. 5.

Improvement of mouse locomotor capacities by Ap₄A treatment after SCI. We used the open field Basso Mouse Scale (BMS) to determine the locomotor skills of spinal cord injured mice after Ap₄A treatment. BMS score (a) revealed that intraperitoneal treatment with Ap₄A (20 mg/Kg) ameliorated the locomotor impairment derived from SCI. Improvements become more evident when locomotion parameters are coded according to the BMS subscore (b) (symbols represent mean ± SEM). The specific analysis of interlimb coordination (c) show that Ap₄A treatment significantly increases the percentage of animals with coordinative capacities 21 days post-injury (DPI; *p < 0.05 in a chi-square test). In agreement, rotarod analysis of coordination and balance of mice at 14 and 21 DPI (d) reveal an increase of the latency time in Ap₄A-treated mice versus vehicle-treated (bars represent the median and dispersion of the sample. *p < 0.05 and ***p < 0.001 vs. vehicle; ^† p < 0.05, ^†† p < 0.01, and ^††† p < 0.001 vs. sham in Student’s t test; n = 6–12)