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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Cancer Res. 2017 Jan 10;15(3):269–280. doi: 10.1158/1541-7786.MCR-16-0227-T

Figure 3. Cisplatin-induced damage is repaired more efficiently in P242R-expressing cells.

Figure 3

A-B. A549 cells expressing WT or P242R were treated with 6.25 μM cisplatin for 24 h and allowed to recover for 0-96 h. Double-strand breaks (A) or apoptotic cells (B) were measured by staining with γH2AX antibody or Annexin V, respectively using flow cytometry. C. XP20S cells deficient in XPA (−XPA; open bars) or complemented with XPA (+XPA; filled bars) expressing WT or P242R were treated with 1.6 μM cisplatin and allowed to recover for 0-48 h. Double-strand breaks were measured by staining with γH2AX antibody using flow cytometry. All data are presented as mean ± SEM (n = 3-4). Two-way ANOVA with Bonferroni’s post hoc test was performed to determine significance.