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. 2017 Mar 3;8:214. doi: 10.3389/fimmu.2017.00214

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Copyright © 2017 Tu, Chen, Zhang and Duan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interleukin (IL)-4 affects the capability of dendritic cells (DCs) in T cell activation through regulating indoleamine 2,3-dioxygenase (IDO) activity. Bone marrow-derived DCs (BMDCs) and monocyte-derived DCs (MoDCs) were generated under various concentration of IL-4 (nanograms per milliliter). Then the BMDCs and MoDCs were cocultured with CFSE-labeled naïve CD4+ T cells from normal C57/BL6 mice or healthy volunteers, respectively, with or without the presence of IDO inhibitor (25 μM). After 5 days, the cells were harvested and analyzed by flow cytometry. (A,B) The CD4+ T cells proliferation was evaluated by cell division. Results are represented as mean ± SD. (C,D) The CD4+ T cells were also collected and analyzed for type 1 cytokines IFN-γ expression by flow cytometry. Results are represented as mean ± SD. The data shown are representative one of the three separate experiments.