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Sterol regulatory element‐binding protein (SREBP) transactivation assay in vitro. HepG2 cells transfected with luciferase vector with SRE were incubated with anagliptin at various concentrations (0.001–10 μmol/L) in medium containing 5% lipoprotein‐deficient serum (LPDS). Data are shown as mean ± standard error of the mean. **P < 0.01, ***P < 0.001 vs control group by Dunnett's test (n = 4). FCS, fetal calf serum.
