Figure 7.

IFN-γ and IL-2 production in suppression cocultures from kidney transplant patients. Cytokines were measured in the supernatant cultures of suppression assays from kidney transplant patients (BLT = 3 and CsA = 4) and controls (n = 4), after a stimulus with beads CD3/CD28 (responder T cells alone) or cocultured with autologous regulatory T cells (Tregs) by 4 days. (A) Responder T cells alone from transplanted individuals produce IFN-γ after CD3/CD28 stimulus in the same way of controls, and this production is reduced in the presence of autologous Tregs. Tregs from BLT-treated patients slightly reduced IFN-γ production by activated T cells. (B) Alterations in IL-2 production were observed in transplanted individuals compared with controls. Responder T cells alone from BLT but not CsA-treated patients produce normal levels of IL-2. Statistical analysis was performed using non-parametric Mann–Whitney test, two tailed. BLT, belatacept; CsA, Cyclosporine A. Control group was composed by healthy individuals.