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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Cancer Res. 2016 Dec 28;15(3):328–339. doi: 10.1158/1541-7786.MCR-16-0257-T

Figure 6.

Figure 6

Ser20 phosphorylation of p53V172F by platinum drugs. (A) p53-knockout A2780 cells transfected with wild type (WT) or mutant (V172F) p53 were treated the next day with cisplatin, DAP or oxaliplatin for 24 hours, then lysed and immunoblotted with the indicated antibodies. (B) 2780CP/Cl-24 cells cultured at 37°C or 32.5°C were treated with cisplatin, DAP or oxaliplatin for 24 hours (37°C and 32.5°C) or 48 hours (32.5°C), harvested and analyzed by Western blot. For p53-Ser20, “short” and “long” exposures were used to visualize the protein.