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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Nature. 2017 Feb 15;543(7643):118–121. doi: 10.1038/nature21407

Figure 2. Stretch-induced mitosis requires the stretch-activated channel Piezo1.

Figure 2

(A) Inhibition or siRNA-mediated knockdown of Piezo1 blocks stretch-induced mitosis, and is rescued with Piezo1-GFP, where values are means of 6 measurements averaged from 5 experiments. (B) Immunoblot confirming knockdown and rescue (SI Fig.1 for full blots). (C) Piezo1 knockdown blocks cell division following wound closure, where graphs are representative of 20 similar videos. (D) CRISPR-mediated Piezo1 knockout reduces Piezo1 mRNA by 50% (n=3) and dramatically decreases mitosis rates, measured in 26 wild type and 53 knockout 4-day old larvae (E, F). Bar = 100μm (images represent 12 total for each). For all graphs, except (C), error bars = s.e.m. P values from an unpaired t-test are **<0.005, ***<0.0005, ****<0.0001.