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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Nature. 2017 Feb 15;543(7643):118–121. doi: 10.1038/nature21407

EDFigure 1. Profile of epithelial cell proliferation and cell density over time.

EDFigure 1

(A) Daily proliferation rates of MDCK cells, as measured by phospho-Histone H3 (H3P) immunostaining, where asterisk indicates when confluence is reached (n=3). Cell division slows but does not stop at day 5. (B), Cell density plateaus by 5 days of growth when cells reach ~11/1000μm2 (n=3). All values are the mean of ten fields from 3 independent experiments with error bars as s.e.m. (C) Cell cycle profiles by FACS at day 1 and day 5 after plating at high density.