EDFigure 4. Piezo1 morphants have reduced cell division in zebrafish epidermis at steady state.

(A) Cell division in zebrafish reaches a low steady state at day 4-5 post-fertilization, where n=50 fish each day and error bars=s.e.m. (B) Photo-activation of zebrafish injected with Piezo1 translation-blocking morpholino results in knockdown of Piezo1 protein, as shown by an immunoblot. Please see SI Figure 1 for scan of full blots. (C, D) Zebrafish Piezo1 morphants have dramatically reduced epidermal mitoses at 5 days post-fertilisation when cells homeostatic growth rate, where values are the averages of the means from 3 separate experiments, error bars are the s.e.m. of the mean, and the P-value is from an unpaired t-test is <0.005. Each micrograph is representative of ~75 samples, bar=100μm.