Fig. 1.

Western blot of the C-terminal (Anti-CSK2) and the N-terminal (Anti-NSK2) antibody directed against the SK2 peptide (a). A band corresponding to the standard SK2 channel isoform was detected at ~52 kD with both antibodies in human and mouse brain tissue. Anti-CSK2 detected three another bands corresponding to two short isoforms and the long isoform of SK2 in human brain. Anti-NSK2 detected signals corresponding to the standard isoform and the long isoform, while the signals for short isoforms were missing. Schematic illustration of Anti-CSK2 and Anti-NSK2 recognition sites (b). hbr human brain, mbr mouse brain, Anti-CSK2 C-terminal SK2 antibody, Anti-NSK2 N-terminal SK2 antibody, Anti-NSK2PB peptide block of Anti-NSK2