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Filipin staining of dermal tissue from the brothers, the sister homozygous for the OSBPL11 c.511C>T (p.(Arg171Trp)) variant (202), the parents and a control sample. The samples of the sibs have free cholesterol-related fluorescent staining in granular pattern (arrows) in dermal fibroblasts interspersed among collagen bundles and ground substance, whereas staining in parents' tissues was less prominent. Granular staining of cholesterol was not observed in the control dermal tissue.
