•The data shown in this article establish the secretion profiles of HO-1 and CD200 in different types of MSCs. •The data analyze the impact of T-cell co-culture on MSC-mediated secretion of HO-1 and CD200. •Constitutive and modulated HO-1 and CD200 secretion levels are differentially observed in these data. •This data helps to better understand the output of the interactions between MSCs and activated T-cells.

•This data will be beneficial for the scientific community focusing on improving the therapeutic capacity of MSCs to modulate the immune response.