Fig. 2.

Relative response intensities of choline and methylation pathway intermediates. E– and E+ embryo (n=15/sample; 4 samples/group) data were normalized against QC sample intensities (n=4) for each individual metabolite. Statistical significance (p<0.05) was calculated using 2-way ANOVA with Sidak’s post-test for multiple comparisons of normalized and natural log-transformed intensity values. Shown are means ± SEM; p-Values are for VitE x Age interactions, unless indicated otherwise. Paired comparison, p-values are indicated as *<0.05, **<0.005, ***<0.001, **** <0.0001.