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. 2017 Feb 21;11:432–441. doi: 10.1016/j.dib.2017.02.046

Fig. 3.

Fig. 3.

Relative response intensities of antioxidant network components from metabolomics and quantification of α-tocopherol and ascorbic acid. A. E– and E+ embryo (n=15/sample; 4 samples/group) relative response data was normalized against QC sample intensities (n=4) for each individual metabolite. B. Quantified levels of α-tocopherol and ascorbic acid, according to established protocols (31) and (33), respectively. Statistical significance (p<0.05) was calculated using 2-way ANOVA with Sidak’s post-test for multiple comparisons of normalized and natural log-transformed intensity values. Shown are means ± SEM; p-Values are for VitE x Age interactions, unless indicated otherwise. Paired comparisons p-values are indicated as *<0.05, **<0.005, ***<0.001, **** <0.0001. C. Antioxidant network scheme showing interaction of antioxidants with lipid radicals and consumption or NADPH.